（一）基础研究：主要集中在纳米酶在治疗药物监测及时检测方面研究

1.课题情况

《抗肿瘤药物TDM 检测试剂盒和生物传感产品关键技术研究》（在研）

《新型纳米酶的制备及其在治疗药物监测应用的基础研究》（NSFC申请项目）

2.指导研究生发表论文情况

※Wu Lulu, Yang Fei, Ma Yuchan, Tang Li, Liu Hongmei, Wang Wenwen, Ouyang Hui, Fu Zhifeng*, He Yong*. Valve-controlled needle-type “mix and detect” integrated distance-based sensor with visual color zoning for point-of-care detection of tacrolimus. Biosens Bioelectron （1区，10.7，返修）

※ Wu LuLu, Zhu Zhongjie, Xue Jinxia, Zheng Liang, Liu Hongmei, Ouyang Hui, Fu Zhifeng*, He Yong*. Chemiluminescent/photothermal dual-mode lateral flow immunoassay based on CoFe PBAs/WS2 nanozyme for rapid and highly sensitive point-of-care testing of gentamicin. Biosens Bioelectron 2024, 265, 116711.（1区，10.7）

※ Zhu Zhongjie, Zhang Yu, Xue Jinxia, Kong Jing, Huang Ling, Ouyang Hui, Fu Zhifeng*, He Yong*. Fluorescent immunochromatographic test strip for therapeutic drug monitoring of methotrexate with high sensitivity and wide dynamic range. Microchimica Acta, 2023, 190:342. (2区, IF 5.7)

（二）药事管理研究：主要集中在横断面研究和中断时间序列研究

1.课题情况

（1）《第二批国家重点监控药品量化指标体系研究：一项多中心横断面研究》（在研）

（2）《DDDs影响因素及变异性研究》（在研）

2.指导研究生发表论文情况

※ Tang Li, Liu Hongmei, Wu Shasha, Liu Jing, Jiang Xiaoyu, Fu Yuanyuan, Tao Na*, He Yong*. A point prevalence survey and monitoring indicators research on 2nd batch of national key monitoring and rational use drugs. Front Pharmacol 2024, 15, 1416010. (2区，IF 4.4)

（三）观察性临床研究：

指导研究生发表论文情况

※ Liu Hongmei, Tang Li, Zheng Liang, Fu Yuanyuan, Qian Mingjiang, Ouyang Canghong, Tao Na, Ou Shuiping, He Yong*. Colistin sulfate versus polymyxin B for the treatment of infections caused by carbapenem-resistant Acinetobacter baumannii: A multicenter retrospective cohort study. Front Pharmacol （2区，4.4，accepted）

主持科研项目：

※ 《DDDs影响因素及变异性研究》，国家卫健委，合同编号：NIHAYS2437，202501-202612

※ 《第二批国家重点监控药品量化指标体系研究：一项多中心横断面研究》，贵州卫健委，合同编号：gzwkj2025-548，202501-202612

※ 《抗肿瘤药物TDM 检测试剂盒和生物传感产品关键技术研究 省科技厅》，贵州省科技厅重点项目，合同编号：黔科合基础-zk[2024]重点0646，202404-202803

※ 贵州省千层次人才项目，贵州省卫健委，202401-202612

※ 《基于噬菌体功能性蛋白识别的抗菌药物敏感性试验新方法》，国家自然科学基金，合同编号：21964023，202001-202312

※ 《基于噬菌体尾丝蛋白识别的侧向层析技术检测细菌的新方法》，贵州省科技厅，合同编号：黔科合基础[2020]1Y044，202001-202212

※ 《铜绿假单胞菌噬菌体PAP1功能性蛋白的重组表达及应用》，博士启动基金，[2018]05号，201901-202112

※ 《医院药学高层次人才引进培养面临的问题及对策研究》，贵州省委组织部，201901-202012

（一）通讯作者或第一作者

※ Wu LuLu, Zhu Zhongjie, Xue Jinxia, Zheng Liang, Liu Hongmei, Ouyang Hui, Fu Zhifeng*, He Yong*. Chemiluminescent/photothermal dual-mode lateral flow immunoassay based on CoFe PBAs/WS₂ nanozyme for rapid and highly sensitive point-of-care testing of gentamicin. Biosens Bioelectron 2024, 265, 116711. （1区 IF 10.7）

※ Tang Li, Liu Hongmei, Wu Shasha, Liu Jing, Jiang Xiaoyu, Fu Yuanyuan, Tao Na*, He Yong*. A point prevalence survey and monitoring indicator research on the second batch of national key monitoring and rational use drugs. Front Pharmacol 2024, 15, 1416010.（2区 IF 4.4）

※ Zhu Zhongjie, Zhang Yu, Xue Jinxia, Kong Jing, Huang Ling, Ouyang Hui, Fu Zhifeng*, He Yong*. Fluorescent immunochromatographic test strip for therapeutic drug monitoring of methotrexate with high sensitivity and wide dynamic range. Microchimica Acta, 2023, 190:342. (2区, IF 5.7)

※ Ma Yuchan, Zhang Yu, Gao Jiaqi, Ouyang Hui, He Yong*, Fu Zhifeng*. PEGylated Ni single-atom catalysts as ultrasensitive electrochemiluminescent probes with favorable aqueous dispersibility for assaying drug-resistant pathogens. Anal. Chem. 2022, 94, 14047-14053. (1区 IF 8.008)

※ Li Jizhou, Xue Jinxia, Zhang Yu, He Yong*, Fu Zhifeng*. Shape-encoded functional hydrogel pellets for multiplexed detection of pathogenic bacteria using a gas pressure sensor. ACS Sens. 2022, 7, 2438-2445. (1区 IF 9.168)

※ Zhang Yu, Li Jizhou, Ma Yuchan, He Yong*, Fu Zhifeng*. Small ubiquitin-related modifier-fused bacteriophage tail fiber protein with favorable aqueous solubility for lateral flow assay of Pseudomonas aeruginosa. Biosens. Bioelectron. 2022, 216, 114637. (1区 IF 12.545)

※ He Yong, Zhao Hang, Liu Yuanwen, Zhou He*. Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition. Sci. Rep. 2021, 11, 11101. (3区IF 4.38)

※ Shi Yanli, He Yong（共同一作）, Zhang Lvxia, Wang Lin, Fu Zhifeng*. Dual-site recognition of Pseudomonas aeruginosa using polymyxin B and bacteriophage tail fiber protein. Anal. Chim. Acta 2021, 1180, 338855. (1区IF 6.56)

※ He Yong, Shi Yanli, Liu Mengli, Wang Yingran, Wang Ling, Lu Shuguang*, Fu Zhifeng*. Nonlytic recombinant phage tail fiber protein for specific recognition of Pseudomonas aeruginosa. Anal. Chem. 2018, 90, 14462-14468. (1区 IF 6.35)

※ He Yong, Wang Yingran, Shi Yanli, Fu Zhifeng*. Molecular recognition strategy for detection and antimicrobial susceptibility testing of Staphylococcus aureus by utilizing teicoplanin and porcine IgG as indicator molecules. Sens. Actuators, B. 2018, 267, 51-57. (1区 IF 6.39)

※ He Yong, Fan Enci, Wang Mengyao, Lu Shuguang*, Fu Zhifeng*. Antimicrobial susceptibility testing by using virulent phages to evaluate bacterial viability. Anal. Methods 2018, 10, 1799-1804. (3区 IF 2.07)

※ He Yong, Wang Mengyao, Fan Enci, Ouyang Hui, Yue Huan, Su Xiaoxiao, Liao Guojian, Wang Ling, Lu Shuguang*, Fu Zhifeng*. Highly specific bacteriophage-affinity strategy for rapid separation and sensitive detection of viable Pseudomonas aeruginosa. Anal. Chem. 2017, 89, 1916-1921. (1区 IF 6.04)

（二）合作

※ Zhou Yali, Wang Pingshi, Xiong Jie, Yue Huan, He Yong, Ouyang Hui, Wang Lin, Fu Zhifeng*. A label-free strategy for measuring the affinity between monoclonal antibody and hapten using microdialysis sampling combined with chemiluminescent detection. Biosens. Bioelectron. 2017, 87, 404-409. (1区IF 8.17)

※ Su Xiaoxiao, Wang Mengyao, Ouyang Hui, Yang Shijia, Wang Wenwen, He Yong, Fu Zhifeng*. Bioluminescent detection of the total amount of viable Gram-positivebacteria isolated by vancomycin-functionalized magnetic particles. Sens. Actuators, B 2017, 241, 255-261. (1区IF 5.67)

※ Wang Mengyao, Wu Yue, He Yong, Su Xiaoxiao, Ouyang Hui, Fu Zhifeng*. Antibiotic-affinity strategy for bioluminescent detection of viable Gram-positive bacteria using daptomycin as recognition agent. Anal. Chim. Acta 2017, 987, 91-97. (1区IF 5.12)

※ Yue Huan, He Yong, Fan Enci, Wang Lin, Lu Suguang, Fu Zhifeng*. Label-free electrochemiluminescent biosensor for rapid and sensitive detection of Pseudomonas aeruginosa using phage as highly specific recognition agent. Biosens. Bioelectron. 2017, 94, 429-432. (1区IF 8.17)

※ Wu Yue, Wang Mengyao, Ouyang Hui, He Yong, Zhao Xiaoyan, Fu Zhifeng*. Teicoplanin-functionalized magnetic beads for detection of Staphylococcus aureus via inhibition of the luminol chemiluminescence by intracellular catalase. Microchim. Acta 2018, 185, 8. (2区IF 5.70)

※ Wang Zhenxing, Ouyang Hui, Tesauro Cinzia, Ottaviani Alessio, He Yong, Fiorani Paola, Xie Hui, Desideri Alessandro, Fu Zhifeng*. Real-time analysis of cleavage and religation activity of human topoisomerase 1 based on ternary fluorescence resonance energy transfer DNA substrate. Arch. Biochem. Biophys. 2018, 643, 1-6. (3区IF 3.12)

※ Su Xiaoxiao, Wang Mengyao, Wu Yue, He Yong, Fu Zhifeng*. Specific chemiluminescent protocol for dual-site recognition of Streptococcus mutans utilizing strong affinity between teicoplanin and Gram-positive bacteria. Talanta 2018, 179, 350-355. (2区IF 4.91)

※ Fan Enci, Peng Jinxiu, He Yong, Wu Yue, Ouyang Hui, Xu Zhaoqing, Fu Zhifeng*. Chemiluminescent analysis of Staphylococcus aureus utilizing phe(11)-protonectin against Gram-positive bacteria. Sens. Actuators, B. 2019, 285, 271-276. (1区 IF 7.1)

※ Shi Yanli, Yang Honglin, He Yong, Fu Zhifeng*. Antibiotic-affinity chromatographic test strip for quantitative analysis and antibiotic resistance testing of Staphylococcus aureus. Talanta 2019, 205, 120130. (2区IF 5.34)

※ Wang Yingran, He Yong, Bhattacharyya Sanjib, Lu Suguang, Fu Zhifeng*. Recombinant Bacteriophage Cell-Binding Domain Proteins for Broad-Spectrum Recognition of Methicillin-Resistant Staphylococcus aureus Strains. Anal. Chem. 2020, 92, 3340-3345. (1区 IF 6.79)